To do this, we tested effects on peroxidase activity, first, with different amounts of the enzyme, next at temperatures of 4oc, room temperature, 32oc, 48oc and boiling then, at ph 3, ph 5, ph 7 and ph 9 and, finally, with the competitive inhibitor, hydroxylamine.
The main enzyme for this lab, peroxidase, is found in many different forms, with optimum phs ranging from 4 to 11 depending on the source and optimum temperatures varying from 10 to 70°c. Report abuse transcript of lab: peroxidase enzyme activity purpose of experiments test how peroxidase enzyme is affected by factors such as enzyme concentration, ph, and temperature measure the effects using guaiacol, a colorless compound with formula c7h8o2 when guaiacol is oxidized, it converts to a dark orange compound called tetraguaiacol.
Sample lab report: factors which affect the activity of the enzyme catalase purpose: must include: background information about concepts involved in the lab, statement of purpose of the lab identification of independent and dependent variables a hypothesis is often not necessary or appropriate.
Transcript of peroxidase lab report the effect of ph on enzyme activity hypothesis the ph will have an effect on enzyme activity as seen through the amount of water displaced, the enzymes in the optimal ph environment of 6 will be the most active, displacing the most water, followed by the basic environment of 9, and the acidic environment of 3 , with the least water displaced. Hydrogen peroxidase is an enzyme found in all aerobic cells, which functions to decompose toxic hydrogen peroxide (petersen and anderson, 2005) the products of this reaction are water and oxygen gas enzymes are complexes produced by living cells, which catalyze chemical reactions in. Enzyme (peroxidase) concentration and enzyme activity (lab report sample) instructions: question description: this is the enzyme lab report, reading and following the word writing a lab report and you can understand what you are going to do for the report.
Knowledge and understanding (concept analysis): enzymes are made of protein, depending on the structure of the amino acid, and the hydrogen and ionic bonds is what makes the difference between the two enzymes (catalase and peroxidase. Enzyme lab report 1 in your own words, define objective qualitative and objective quantitative data in your own words, define objective qualitative and objective quantitative data do not use examples as your sole definition. Documents similar to biology 12 peroxidase experiment skip carousel carousel previous carousel next exp 4 restriction enzyme uploaded by peter hong leong cheah ap biology enzyme lab report uploaded by patrick peroxidase lab uploaded by ryan zamora determining the properties of an enzyme uploaded by geoffreit.
Peroxidase is an enzyme that is essential in removing hydrogen peroxide which is a byproduct in some metabolic reactions students of biology 1406-83003 class at richland college conducted an enzyme lab to determine the effects of different factors on enzymes. Lab report: enzymes - free download as word doc (doc), pdf file (pdf), text file (txt) or read online for free how enzymes work is fascinating take a look at this lab report for a better understanding of how enzymes work.