The number of mice and methods for housing, breeding, and manipulating the mice for the purpose of generating transgenic mice must be approved by the appropriate institutional animal care and use committee (iacuc) and conform to governmental regulations. Transgenic mice are often obtained by mating the first generation hybrid mice, which are in actuality a cross between two inbred strains if brother–sister mating is used to maintain the transgenic line, then a recombinant inbred strain is created, which is actually a hybrid of the two parental strains. In the years since, these transgenic and knockout mice have become commonplace in the laboratory capecchi's pioneering work in gene targeting has taught us much about how the body builds—and rebuilds—itself he has given scientists worldwide the tools to make important discoveries about human diseases, from cancer to obesity.
The transgenic mice, which now number nearly 500, were derived from six founder lines that contain a chimeric gene in which a copy of the cdna for pepck-c was linked to the skeletal actin gene promoter, containing the 3'-end of the bovine growth hormone gene. Supermouse was created about four years ago by injecting a highly active form of a gene for an enzyme called phosphonenolpyruvate carboxykinase (pepck-c) int. These super idol transgenic mice express human mylip /idol modified with k293r, k309r, k310r, and k320r mutations under the control of the liver-specific mouse albumin promoter liver-selective transgenic mice are a robust, dominantly inherited, diet-inducible model for the study of artherosclerosis.
Supermouse was created about four years ago by injecting a highly active form of a gene for an enzyme called phosphonenolpyruvate carboxykinase (pepck-c) into a mouse embryo. A genetically modified mouse (mus musculus) is a mouse that has had its genome altered through the use of genetic engineering techniques genetically modified mice are commonly used for research or as animal models of human diseases, and are also used for research on genes.
A genetically engineered super mouse can run over 4 hours with no break, eat more but doesn't get fat, has more sex, live longer, 10 times more active than n. Transgenic mice mario r capecchi, phd, of the university of utah, won the 2007 nobel prize in physiology or medicine capecchi shared the prize with oliver smithies of university of north carolina, chapel hill, and sir martin evans of cardiff university in the uk. Transgenic mice in particular have been used to study cancer, obesity, heart disease, arthritis, anxiety and many other disorders who created the first transgenic animal dr rudolf jaenisch created the first transgenic mouse in 1974 by introducing foreign dna into the mouse embryo.
Workflow of transgenic mice services each full transgenic project is split into the following phases: 1 transgenic strategy design design a transgenic strategy that meets your research needs develop a pcr genotyping strategy for founder screening 2 transgenic vector construction.